In recent years, growing attention has been devoted to cold adapted microorganisms and their enzymes. A gene library was constructed using the genome of a new aerobic psychrotolerant marine isolate Pseudoalteromonas arctica sp. nov. The gene encoding an esterase was identified by screening the library on tributyrin plates. The recombinant protein was purified (3,3 - fold) in one step using a Ni- column and was subsequently characterised. The enzyme has unique properties since it requires a very low activation energy and retains 50% of its maximal activity at the freezing point of water.
| Autorius: | Rami Al Khudary |
| Leidėjas: | BoD - Books on Demand |
| Išleidimo metai: | 2006 |
| Knygos puslapių skaičius: | 136 |
| ISBN-10: | 3833466448 |
| ISBN-13: | 9783833466441 |
| Formatas: | Knyga minkštu viršeliu |
| Kalba: | Anglų |
| Žanras: | Microbiology (non-medical) |
Parašykite atsiliepimą apie „A Novel Cold Active Esterase from the Marine Psychrotolerant Isolate Pseudoalteromonas Arctica sp. nov.“
