Withania somnifera Dunal (Ashwagandha) is an Indian medicinal plant with significant pharmacological properties like adaptogenic, antioxidant, hypoglycemic and anti-stroke effects. Several of its chemotypes includes NMITLI-101, NMITLI-118, NMITLI-128. The present work elaborates phytochemical and analytical studies on NMITLI118RT+ a candidate drug under the CSIR umbrella (derived from a new chemotype of W. somnifera). Here we report the development validation and applications of a simple, rapid and reliable isocratic HPLC method for quantification of Withanolide A in NMITLI118RT+ on a CN column utilizing gliclazide as an internal standard which could detect Withanolide A at about 3.8 min and gliclazide (internal standard) at about 4.8 min with a linearity range of 2-20 µg/mL. A second method here reports the development and validation of a simple isocratic method for simultaneous estimation of Withanolide A and Withanone (sesquiterpene lactone markers) established on a C-18; 3µ column which could detect Withanolide A and Withanone at retention times of about 20.7 min and 21.6 min respectively in the linearity range of 2-50 µg/mL for both.
| Autorius: | Hafsa Ahmad, Anil Dwivedi, Sarvesh Paliwal, |
| Leidėjas: | LAP LAMBERT Academic Publishing |
| Išleidimo metai: | 2017 |
| Knygos puslapių skaičius: | 52 |
| ISBN-10: | 3330343206 |
| ISBN-13: | 9783330343207 |
| Formatas: | Knyga minkštu viršeliu |
| Kalba: | Anglų |
| Žanras: | Pharmacology |
Parašykite atsiliepimą apie „Analytical studies on a standardized extract of Withania somnifera L.“
