Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3¿-modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3¿-modification) that the DNA-synthesis can continue (i. e. ¿reversible¿ termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3¿-modified nucleosides as key compounds for these terminators and the evaluation of suitable 3¿-modification cleavage conditions.
| Autorius: | Angelika Keller |
| Leidėjas: | Südwestdeutscher Verlag für Hochschulschriften AG Co. KG |
| Išleidimo metai: | 2015 |
| Knygos puslapių skaičius: | 240 |
| ISBN-10: | 3838113551 |
| ISBN-13: | 9783838113555 |
| Formatas: | Knyga minkštu viršeliu |
| Kalba: | Anglų |
| Žanras: | Organic chemistry |
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